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Lisa-Marie Schmitz Foto von Lisa-Marie Schmitz

(+49)231 755-7391


Raum G1 6.25


Cytochrome P450 monooxygenases (P450s) catalyze oxidation reactions on a broad range of substrates leading to many different chemical transformations of their substrates. As they can modify and diversify chemical compounds acting in a regio- and often stereoselective manner, they are considered powerful biocatalysts for the production of high value oxychemicals, such as pharmacological compounds. Due to their natural roles in detoxification reactions and metabolite biosynthetic pathways, P450 monooxygenases are found in nearly all kingdoms of life. Consequently, there is still a high potential to find novel P450s with a new substrate and reaction spectrum.
The objective of the project is to identify novel microbial P450s using a combination of a bioinformatics approach and activity screening. Characterization and optimization of both wildtype strains and heterologous expressed P450s can yield in the development of novel hydroxylation processes for biotechnological applications.

Figure: Genome mining combined with high throughput screening and optimization to establish biocatalysts for hydroxylation processes



  • Schmitz LM., Rosenthal K., Lütz S. (2019)
    Recent Advances in Heme Biocatalysis Engineering
    Biotechnology and Bioengineering DOI

  • Schmitz LM., Schäper J., Rosenthal K., Lütz S. (2019)
    Accessing the biocatalytic potential for C-H-activation by targeted genome mining and screening
    ChemCatChem DOI

  • Schmitz LM, Rosenthal K, and Lütz S (2017)
    Enzyme-based electrobiotechnological synthesis
    In: Advances in Biochemical Engineering/Biotechnology. Springer, Berlin, Heidelberg

  • Zambanini T, Hartmann SK, Schmitz LM, Büttner L, Hosseinpour Tehrani H, Geiser E, Beudels M, Venc D, Wandrey G, Büchs J, Schwarzländer M, Blank LM, Wierckx N (2017)
    Promoters from the itaconate cluster of Ustilago maydis are induced by nitrogen depletion
    Fungal Biology and Biotechnology 4(1): p. 11


  • LM. Schmitz, J. Schäper, K. Rosenthal, S, Lütz
    Identification of novel microbial P450s as drug-metabolizing enzymes
    Basellife, 9.-12. September 2019, Basel, Switzerland

  • Schmitz LM, Schäper J, Lütz S (2019)
    Genome mining combined with high throughput screening revealed novel biocatalysts for hydroxylation reactions
    Trends in Enzymology and Biocatalysis, 27-31 May, Rome, Italy

  • Schmitz LM, Buchholz P, Pleiss J, Lütz S (2017)
    Identifying potential novel biocatalysts for hydroxylation processes by genome mining
    BioProcessingDays 2017, 20. - 22. Februar 2017, Recklinghausen, Germany

  • Schmitz LM, Lütz S (2017)
    Identification of novel biocatalysts for hydroxylation processes
    20th International Conference on Cytochrome P450, 27. -31. August 2017, Düsseldorf, Germany